Abstract
Beta-glucan,
one of the major cell wall components of Saccharomyces cerevisiae (S.
cerevisiae), has been found to enhance immune functions.
At present
study, we developed an optimal procedure to extract and purify
beta-glucan. At first, yeast cells were grown in sabouraud dextrose agar
and then cultured in yeast extract-peptone-glucose (YPG) broth. After
incubation, cells were harvested, washed and disrupted by means of
sonication method. The obtained cell walls were used to prepare
alkali-soluble beta-glucan (glucan-S1). In this regard, 2% sodium
hydroxide (NaOH) and 3% acetic acid were used in alkaline-acid
extraction, respectively.
This preparation contained 2.4% protein. In
the next step, DEAE sephacel chromatography was used to remove remaining
proteins (glucan-S2). Subsequently this preparation was applied into
concanavalin-A sepharose column to remove manann. Finally, beta-glucan
free of mannoprotein complexes was prepared (glucan-S3).
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